Siglec-3/CD33 Antibody (BLR061G)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77356

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-77356
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BLR061G

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Siglec-3/CD33 Antibodies »

Product Specifications

Immunogen

Immunogen was a peptide representing a region between residue 314 and the C-terminus (residue 364) of human Myeloid cell surface antigen CD33 using the numbering given in entry NP_001763.3 (Gene ID 945).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Siglec-3/CD33 Antibody (BLR061G)

Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356]

Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356]

Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Antibody: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.
Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Section of Acute Myeloid Leukemia (AML). Antibody: Rabbit anti-CD33 recombinant monoclonal antibody [BLR061G] (A700-061 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Immunoprecipitation: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356]

Immunoprecipitation: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356]

Immunoprecipitation: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Detection of human CD33 by WB of immunoprecipitates from HEL 92.1.7 lysate. Antibodies: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356) and rabbit anti-CD33 recombinant monoclonal [BL-302-3E10].

Applications for Siglec-3/CD33 Antibody (BLR061G)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

20 ul/mg lysate

Western Blot

1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE sections.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Siglec-3/CD33

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody (BLR061G)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Siglec-3/CD33 Antibody (BLR061G)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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