Siglec-3/CD33 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29619

Novus Biologicals, part of Bio-Techne
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NBP2-29619
NBP2-29619SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Siglec-3/CD33 Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal portion of human CD33 protein (between residues 30-200) [UniProt P20138]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The canonical form of CD33 is 364 amino acids long (~39.8 kDa) and it is processed via cleavage of signal peptide (n-terminal amino acids 1-17). This protein is a single-pass type I membrane protein with an extracellular domain as well as a cytoplasmic tail, so that it is expected to produce a membrane-cytoplasmic pattern in immunostaining assays.

Scientific Data Images for Siglec-3/CD33 Antibody - BSA Free

Western Blot: Siglec-3/CD33 Antibody [NBP2-29619]

Western Blot: Siglec-3/CD33 Antibody [NBP2-29619]

Western Blot: Siglec-3/CD33 Antibody [NBP2-29619] - Analysis of CD33 in the partial recombinant protein.
Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-29619]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-29619]

Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-29619] - Analysis of CD33 protein in a section of human small intestinal malignant stromal tumor using 5 ug/mL concentration of CD33 antibody. The cancer cells developed the expected membrane-cytoplasmic staining pattern.

Applications for Siglec-3/CD33 Antibody - BSA Free

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 27535972)

Flow Cytometry

reported in scientific literature (PMID 27535972)

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Western Blot

1 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-3/CD33

CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Entrez Gene IDs

945 (Human)

Gene Symbol

CD33

UniProt

P20138

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Siglec-3/CD33 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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