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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE/Cy7 (Excitation = 488 nm, Emission = 778 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 191240

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Siglec-9
Gln18-Gly348 (predicted)
Accession # Q9Y336

Specificity

Detects human Siglec-9 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouseSiglec‑E is observed and less than 1% cross-reactivity with recombinant human (rh) Siglec-3, rhSiglec-5, rhSiglec-6, rhSiglec-7, rhSiglec-8 or rhSiglec-10 is observed. In Western blots, approximately 100% cross-reactivity with recombinant mouse Siglec‑E is observed undernon-reduced conditions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Siglec-9 Antibody (191240) [PE/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Siglec-9

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface

Long Name

Sialic Acid Binding Ig-like Lectin 9

Alternate Names

CD329, OBBP-Like, Siglec9

Gene Symbol

SIGLEC9

Additional Siglec-9 Products

Product Documents for Siglec-9 Antibody (191240) [PE/Cy7]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Siglec-9 Antibody (191240) [PE/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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