Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Sirtuin 2/SIRT2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human SIRT2. The immunogen is located within the first 50 amino acids of SIRT2. Amino Acid Squence: AGKVQEAQDSDSDSEGG

Marker

Early Oligodendrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sirtuin 2/SIRT2 Antibody - BSA Free

Western Blot: Sirtuin 2/SIRT2 AntibodyBSA Free [NBP1-76880]

Western Blot: Sirtuin 2/SIRT2 AntibodyBSA Free [NBP1-76880]

Western Blot: Sirtuin 2/SIRT2 Antibody [NBP1-76880] - Human brain lysate with SIRT2 antibody at (A) 2.5 and (B) 5 ug/mL.
Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76880]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76880]

Immunohistochemistry-Paraffin: Sirtuin 2/SIRT2 Antibody [NBP1-76880] - Human brain tissue using Sirt2 antibody at 2.5 ug/ml.
Sirtuin 2/SIRT2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76880] -

Immunocytochemistry/ Immunofluorescence: Sirtuin 2/SIRT2 Antibody - BSA Free [NBP1-76880] - Immunofluorescence of sirt2 in human brain tissue with sirt2 antibody at 20 ug/mL.

Applications for Sirtuin 2/SIRT2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

2.5-5 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 2/SIRT2

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Gene Symbol

SIRT2

UniProt

NP_036369 (Human)

Additional Sirtuin 2/SIRT2 Products

Product Documents for Sirtuin 2/SIRT2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Sirtuin 2/SIRT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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