Sirtuin 2/SIRT2 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76880CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-76880AF350

Alexa Fluor 405

NBP1-76880AF405

Alexa Fluor 488

NBP1-76880AF488

Alexa Fluor 532

NBP1-76880AF532

Alexa Fluor 594

NBP1-76880AF594

Alexa Fluor 647

NBP1-76880AF647

Alexa Fluor 700

NBP1-76880AF700

Alexa Fluor 750

NBP1-76880AF750

Biotin

NBP1-76880B

DyLight 350

NBP1-76880UV

DyLight 405

NBP1-76880V

DyLight 488

NBP1-76880G

DyLight 550

NBP1-76880R

DyLight 594

NBP1-76880DL594

DyLight 650

NBP1-76880C

DyLight 680

NBP1-76880FR

DyLight 755

NBP1-76880IR

FITC

NBP1-76880F

HRP

NBP1-76880H

Janelia Fluor 525

NBP1-76880JF525

Janelia Fluor 549

NBP1-76880JF549

Janelia Fluor 646

NBP1-76880JF646

Janelia Fluor 669

NBP1-76880JF669

mFluor Violet 450 SE

NBP1-76880MFV450

mFluor Violet 500 SE

NBP1-76880MFV500

mFluor Violet 610 SE

NBP1-76880MFV610

PerCP

NBP1-76880PCP

Forumulation

Catalog #

BSA Free

NBP1-76880

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sirtuin 2/SIRT2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human SIRT2. The immunogen is located within the first 50 amino acids of SIRT2. Amino Acid Squence: AGKVQEAQDSDSDSEGG

Marker

Early Oligodendrocyte Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Sirtuin 2/SIRT2 Antibody [CoraFluor™ 1]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Sirtuin 2/SIRT2

Silent information regulator 2 (Sir2) family of enzymes has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging. O-acetyl-ADP-ribose causes a delay/block in oocyte maturation and results in a delay/block in embryo cell division in blastomeres. It has been demonstrated that the production of O-acetyl-ADP-ribose is evolutionarily conserved among Sir2-like enzymes from yeast, Drosophila, and human. Also, endogenous yeast Sir2 complex from telomeres was shown to generate O-acetyl-ADP-ribose (1). SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. Knockdown of SIRT2 via siRNA results in tubulin hyperacetylation. SIRT2 colocalizes and interacts in vivo with HDAC6, another tubulin deacetylase (2). Cytoskeleton-related protein, SIRT2 is down-regulated in gliomas, and data suggests that ectopic expression of SIRT2 in glioma cell lines led to the perturbation of the microtubule network (3).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2L2, SIRT2

Gene Symbol

SIRT2

Additional Sirtuin 2/SIRT2 Products

Product Documents for Sirtuin 2/SIRT2 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Sirtuin 2/SIRT2 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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