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Smad2 [p Ser467] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54771

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Smad2 (Phospho-Ser467) antibody was raised against a peptide sequence around phosphorylation site of serine 467 (C-S-S-M-S (p) ) derived from Human Smad2.

Modification

p Ser467

Specificity

This antibody detects endogenous level of Smad2 only when phosphorylated at serine 467.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Smad2 [p Ser467] Antibody - BSA Free

Western Blot: Smad2 [p Ser467] AntibodyBSA Free [NBP2-54771]

Western Blot: Smad2 [p Ser467] AntibodyBSA Free [NBP2-54771]

Western Blot: Smad2 [p Ser467] Antibody [NBP2-54771] - Analysis of lysed extracts from HeLa cells untreated or treated with PMA using Smad2 (Phospho-Ser467).

Applications for Smad2 [p Ser467] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Epitope affinity purified

Formulation

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: Smad2

Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox, pSMAD

Entrez Gene IDs

NP_001003652.1 (Human)

Gene Symbol

SMAD2

Additional Smad2 Products

Product Documents for Smad2 [p Ser467] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Smad2 [p Ser467] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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