Smad4 Antibody (SMAD/6309R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14116
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # SMAD/6309R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human full-length (aa1-552) Smad4 protein (Uniprot: Q13485 )
Localization
Nucleus, Cytoplasm, Cytoplasmic in absence of ligand, Migrates to nucleus when complexed with R-SMAD
Marker
Pancreatic Adenocarcinoma Marker / Tumor Suppressor
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13963).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Smad4 Antibody (SMAD/6309R) - Azide and BSA Free
Smad4 Antibody (SMAD/6309R)
Formalin-fixed, paraffin-embedded human small intestine stained with Smad4 antibody (SMAD/6309R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for Smad4 Antibody (SMAD/6309R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Smad4
Long Name
Mothers Against DPP Homolog 4
Alternate Names
DPC4, MADH4
Gene Symbol
SMAD4
Additional Smad4 Products
Product Documents for Smad4 Antibody (SMAD/6309R) - Azide and BSA Free
Product Specific Notices for Smad4 Antibody (SMAD/6309R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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