Sonic Hedgehog/Shh Antibody (05)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06169

Novus Biologicals, part of Bio-Techne
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NBP3-06169-100ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 05

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Sonic Hedgehog/Shh Antibodies »

Product Summary for Sonic Hedgehog/Shh Antibody (05)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Sonic Hedgehog/Shh (Uniprot#: Q15465; Met1-Gly197).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Applications for Sonic Hedgehog/Shh Antibody (05)

Application
Recommended Usage

ELISA

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).

Alternate Names

HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS

Gene Symbol

SHH

Additional Sonic Hedgehog/Shh Products

Product Documents for Sonic Hedgehog/Shh Antibody (05)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sonic Hedgehog/Shh Antibody (05)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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