Spectrin alpha 1 Antibody (SPTA1/1832)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79772

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SPTA1/1832

Concentration

0.2 mg/ml

View all Spectrin alpha 1 Antibodies »

Product Summary for Spectrin alpha 1 Antibody (SPTA1/1832)

Immunogen

Recombinant human Spectrin alpha 1 fragment (around aa356-475) (exact sequence is proprietary) (Uniprot: P02549)

Localization

Cytoplasmic, Cell Surface

Marker

Erythrocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79906)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Spectrin alpha 1 Antibody (SPTA1/1832)

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772]

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772]

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772] - Western Blot Analysis of Human HeLa cell lysate using Spectrin alpha 1 Antibody (SPTA1/1832).
Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772] - Formalin-fixed, paraffin-embedded human Tonsil stained with Spectrin alpha 1 Antibody (SPTA1/1832).
Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772]

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772]

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) [NBP2-79772] - Western Blot Analysis of Human K562 cell lysate using Spectrin alpha 1 Antibody (SPTA1/1832).
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Applications for Spectrin alpha 1 Antibody (SPTA1/1832)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Spectrin alpha 1

Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface and transmembrane movement of phospholipids and cholesterol. On SDS PAGE gels erythrocyte spectrin appears as two bands referred to as alpha (240 kDa) and beta (220 kDa). While the simplest form of spectrin in solution is an alpha-beta heterodimer, spectrin can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of spectrin-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Alternate Names

alpha-I spectrin, EL2, erythrocyte, HS3, spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Gene Symbol

SPTA1

UniProt

P02549 (Human)

Additional Spectrin alpha 1 Products

Product Documents for Spectrin alpha 1 Antibody (SPTA1/1832)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Spectrin alpha 1 Antibody (SPTA1/1832)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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