Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SPTA1/1832

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Spectrin alpha 1 Antibodies »

Product Specifications

Immunogen

Recombinant human Spectrin alpha 1 fragment (around aa356-475) (exact sequence is proprietary) (Uniprot: P02549)

Localization

Cytoplasmic, Cell Surface

Marker

Erythrocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

240 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79772).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832)Azide and BSA Free [NBP2-79906]

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832)Azide and BSA Free [NBP2-79906]

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Western Blot Analysis of Human HeLa cell lysate using Spectrin alpha 1 Mouse Monoclonal Antibody (SPTA1/1832).
Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906]

Immunohistochemistry-Paraffin: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Formalin-fixed, paraffin-embedded human Tonsil stained with Spectrin alpha 1 (SPTA1) Mouse Monoclonal Antibody (SPTA1/1832).
Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832)Azide and BSA Free [NBP2-79906]

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832)Azide and BSA Free [NBP2-79906]

Western Blot: Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free [NBP2-79906] - Western Blot Analysis of Human K562 cell lysate using Spectrin alpha 1 Mouse Monoclonal Antibody (SPTA1/1832).
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Applications for Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Spectrin alpha 1

Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface and transmembrane movement of phospholipids and cholesterol. On SDS PAGE gels erythrocyte spectrin appears as two bands referred to as alpha (240 kDa) and beta (220 kDa). While the simplest form of spectrin in solution is an alpha-beta heterodimer, spectrin can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of spectrin-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Alternate Names

alpha-I spectrin, EL2, erythrocyte, HS3, spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Gene Symbol

SPTA1

Additional Spectrin alpha 1 Products

Product Documents for Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Spectrin alpha 1 Antibody (SPTA1/1832) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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