SREBP1 Antibody (2A4) [DyLight 650]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-582C

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Chicken, Golden Syrian Hamster, Hamster, Monkey

Applications

Immunohistochemistry-Frozen, Knockout Validated, Simple Western, Western Blot

Label

DyLight 650 (Excitation = 652 nm, Emission = 672 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SREBP1 Antibodies »

Product Specifications

Immunogen

6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 23720350). Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature. Monkey reactivity reported in scientific literature (PMID: 26437365).

Localization

Cytoplasmic and nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for SREBP1 Antibody (2A4) [DyLight 650]

Application
Recommended Usage

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: SREBP1

The importance of lipids for survival is underscored when starvation occurs - cellular responses occur in major organ pathways to protect against detrimental loss of lipids. For example, lipid generation and stabilization occurs in the brain even when serious deficiencies of dietary lipid exist. Embryonic and fetal tissues also are spared the effects of lacking lipids, even at the expense of maternal health. In all of these examples, the transcription factor Sterol-Regulatory Element-Binding Protein 1 (SREBP1) is a critical contributor to effective cholesterol and fatty acid synthesis, through the PPAR-gamma and AMPK signaling pathways. During fasting and starvation, more than 40% of transcription factors in an individual are dedicated to adipogenesis. However, in certain conditions that lead to obesity, the SREBP1 transcription activity is overstimulated by elevated cofactor expression (as in the case of resistin) and the result is erroneous de novo lipogenesis in hepatocytes and plasma. Excessive lipogenesis in these tissues is then associated with dyslipidemia, atherosclerosis and abnormal HDL/LDL/VLDL ratios and commonly attributed obesity-related disease.

Long Name

Sterol regulatory element-binding protein 1

Alternate Names

bHLHd1, SREBF1, SREBP-1

Gene Symbol

SREBF1

Additional SREBP1 Products

Product Documents for SREBP1 Antibody (2A4) [DyLight 650]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SREBP1 Antibody (2A4) [DyLight 650]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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