SREBP1 Antibody (2A4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80959
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat, Canine, Chicken, Golden Syrian Hamster, Hamster, Monkey
Applications
Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2A4
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
6 His-tag fusion protein of human SREBP1 corresponding to amino acids 301-407. [UniProt# P36956]
Reactivity Notes
Canine reactivity reported in scientific literature (PMID: 23720350). Hamster reactivity reported in scientific literature (PMID: 24393244). Chicken reactivity reported in multiple pieces scientific literature. Monkey reactivity reported in scientific literature (PMID: 26437365).
Localization
Cytoplasmic and nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for SREBP1 Antibody (2A4) - Azide and BSA Free
Western Blot: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]
Western Blot: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY license. Image from the standard format of this antibody.Western Blot: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]
Western Blot: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa). Image from the standard format of this antibody.Simple Western: SREBP1 Antibody (2A4)Azide and BSA Free [NBP2-80959]
Simple Western: SREBP1 Antibody (2A4) - Azide and BSA Free [NBP2-80959] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.Applications for SREBP1 Antibody (2A4) - Azide and BSA Free
Application
Recommended Usage
Simple Western
1:12.5
Western Blot
1-2ug/ml
Application Notes
This SREBP1 (clone 2A4) antibody is useful for WB where a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SREBP1
Long Name
Sterol regulatory element-binding protein 1
Alternate Names
bHLHd1, SREBF1, SREBP-1
Gene Symbol
SREBF1
Additional SREBP1 Products
Product Documents for SREBP1 Antibody (2A4) - Azide and BSA Free
Product Specific Notices for SREBP1 Antibody (2A4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...