SSEA-1 Antibody (FUT4/1478R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49878

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # FUT4/1478R

Concentration

0.2 mg/ml

View all SSEA-1 Antibodies »

Product Summary for SSEA-1 Antibody (FUT4/1478R)

Immunogen

Recombinant human full-length SSEA-1 protein (Uniprot: P22083)

Localization

Cell surface and granular paranuclear

Marker

Reed-Sternberg Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-49879)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for SSEA-1 Antibody (FUT4/1478R)

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878]

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878]

Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878] - Staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot. Image using the Azide and BSA Free format of this antibody.
Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878]

Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878]

Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878] - human Hodgkin's Lymphoma stained with CD15 Rabbit Monoclonal Antibody (FUT4/1478R).
Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878]

Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878]

Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) [NBP2-49878] - Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red). Image using the Azide and BSA Free format of this antibody.

Applications for SSEA-1 Antibody (FUT4/1478R)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: SSEA-1

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Gene Symbol

FUT4

UniProt

P22083 (Human)

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (FUT4/1478R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SSEA-1 Antibody (FUT4/1478R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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