Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # FUT4/1478R

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all SSEA-1 Antibodies »

Product Summary for SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free

Immunogen

Recombinant human full-length SSEA-1 protein (Uniprot: P22083)

Localization

Cell surface and granular paranuclear

Marker

Reed-Sternberg Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified by Protein A Column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-49878).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879]

Immunocytochemistry/ Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879]

Immunocytochemistry/Immunofluorescence: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879]

Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879]

Immunohistochemistry-Paraffin: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R).
Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879]

Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879]

Flow Cytometry: SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free [NBP2-49879] - Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Applications for SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 8.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: SSEA-1

SSEA1 (stage-specific mouse embryonic antigen) is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. SSEA-1 is also known as CD15, although SSEA1 is more commonly used in a stem cell research context because SSEA-1 refers to the marker identified specifically by monoclonal antibody, clone MC-480. Clone MC-480 is now the gold standard reagent for mouse embryonic stem cells. Only mouse ESCs express SSEA1; human SSEA1 only stains cells after differentiation.

Long Name

Stage-specific Embryonic Antigen-1

Alternate Names

SSEA1

Gene Symbol

FUT4

Additional SSEA-1 Products

Product Documents for SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SSEA-1 Antibody (FUT4/1478R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.