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ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07770

Clone ZB55 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP3-07770-100ug
NBP3-07770-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # ZB55

Concentration

0.2 mg/ml

Product Summary for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55)

Immunogen

ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 peptides (Uniprot: P15907)

Localization

Cell surface and cytoplasmic

Specificity

Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This monoclonal antibody reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08436)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55)

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770] - Formalin-fixed, paraffin-embedded normal human spleen tissue stained with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Mouse Monoclonal Antibody (ZB55).
Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770]

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) [NBP3-07770] - Formalin-fixed, paraffin-embedded normal human spleen tissue stained with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Mouse Monoclonal Antibody (ZB55).

Applications for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

PEG purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ST6 Gal Sialyltransferase 1/ST6GAL1

CD75 is encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. This antibody recognizes the CD75 cell surface antigen, an alpha 2,6 sialyated carbohydrate epitope expressed by mature B cells, especially germinal centre B cells, red blood cells and some epithelial cells.

Long Name

ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1

Alternate Names

Alpha 2,6-ST, B-cell antigen CD75, CD75, SIAT1, ST6N

Gene Symbol

ST6GAL1

UniProt

Additional ST6 Gal Sialyltransferase 1/ST6GAL1 Products

Product Documents for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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