ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08436
Clone ZB55 was used by HLDA to establish CD designation.
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # ZB55
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 peptides (Uniprot: P15907)
Localization
Cell surface and cytoplasmic
Specificity
Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This monoclonal antibody reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07770).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images
Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) - Azide and BSA Free [NBP3-08436]
Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) - Azide and BSA Free [NBP3-08436] - Formalin-fixed, paraffin-embedded normal human spleen tissue stained with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Mouse Monoclonal Antibody (ZB55).Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) - Azide and BSA Free [NBP3-08436]
Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (ZB55) - Azide and BSA Free [NBP3-08436] - Formalin-fixed, paraffin-embedded normal human spleen tissue stained with ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Mouse Monoclonal Antibody (ZB55).Applications
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
PEG purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: ST6 Gal Sialyltransferase 1/ST6GAL1
Long Name
ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1
Alternate Names
Alpha 2,6-ST, B-cell antigen CD75, CD75, SIAT1, ST6N
Gene Symbol
ST6GAL1
Additional ST6 Gal Sialyltransferase 1/ST6GAL1 Products
- All Products for ST6 Gal Sialyltransferase 1/ST6GAL1
- ST6 Gal Sialyltransferase 1/ST6GAL1 cDNA Clones
- ST6 Gal Sialyltransferase 1/ST6GAL1 ELISA Kits
- ST6 Gal Sialyltransferase 1/ST6GAL1 Lysates
- ST6 Gal Sialyltransferase 1/ST6GAL1 Primary Antibodies
- ST6 Gal Sialyltransferase 1/ST6GAL1 Proteins and Enzymes
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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