STAT5A Antibody (OTI9F7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00622

Novus Biologicals, part of Bio-Techne
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NBP2-00622
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9F7

Concentration

0.73 mg/ml

View all STAT5a Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

90.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT5A Antibody (OTI9F7)

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622]

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622]

Western Blot: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of extracts (35ug) from 9 different cell lines by using anti-STAT5A monoclonal antibody.
Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622]

Immunohistochemistry-Paraffin: STAT5A Antibody (OTI9F7) [NBP2-00622] - Staining of paraffin-embedded Human prostate tissue using anti-STAT5A mouse monoclonal antibody.
Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622]

Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622]

Flow Cytometry: STAT5A Antibody (OTI9F7) [NBP2-00622] - Analysis of Jurkat cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).
View 8 more images

Applications for STAT5A Antibody (OTI9F7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NBP2-00622 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.73 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT5a

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq].

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Entrez Gene IDs

6776 (Human)

Gene Symbol

STAT5A

Additional STAT5a Products

Product Documents for STAT5A Antibody (OTI9F7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STAT5A Antibody (OTI9F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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