STAT5A Antibody (OTI9F7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74392

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI9F7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all STAT5a Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human STAT5A (NP_003143) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

90.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT5A Antibody (OTI9F7) - Azide and BSA Free

Western Blot: STAT5A Antibody (OTI9F7)Azide and BSA Free [NBP2-74392]

Western Blot: STAT5A Antibody (OTI9F7)Azide and BSA Free [NBP2-74392]

Western Blot: STAT5A Antibody (OTI9F7) - Azide and BSA Free [NBP2-74392] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT5A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT5A.
Immunohistochemistry: STAT5A Antibody (OTI9F7) - Azide and BSA Free [NBP2-74392]

Immunohistochemistry: STAT5A Antibody (OTI9F7) - Azide and BSA Free [NBP2-74392]

Immunohistochemistry: STAT5A Antibody (OTI9F7) - Azide and BSA Free [NBP2-74392] - Staining of paraffin-embedded Human lymphoma tissue using anti-STAT5A mouse monoclonal antibody.
Flow Cytometry: STAT5A Antibody (OTI9F7) - Azide and BSA Free [NBP2-74392]

Flow Cytometry: STAT5A Antibody (OTI9F7) - Azide and BSA Free [NBP2-74392]

Flow Cytometry: STAT5A Antibody (OTI9F7) - Azide and BSA Free [NBP2-74392] - Analysis of Jurkat cells, using anti-STAT5A antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for STAT5A Antibody (OTI9F7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT5a

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Gene Symbol

STAT5A

Additional STAT5a Products

Product Documents for STAT5A Antibody (OTI9F7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for STAT5A Antibody (OTI9F7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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