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STIM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44168

Novus Biologicals, part of Bio-Techne
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NBP2-44168
NBP2-44168-0.05mg

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

STIM1 Antibody was prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the N-terminus of human STIM1. (Uniprot: Q13586)

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with Stim I from other sources has not been determined.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Description

This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for STIM1 Antibody

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168] - Staining of human thyroid tissue
Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168]

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168] - Staining of human testis tissue.
STIM1 Antibody

STIM1 Antibody

Immunohistochemistry of Sheep STIM1 Antibody. Tissue: Thyroid. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GOK / STIM1 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase sheep secondary antibody at 1:10,000 for 45 min at RT. Staining: GOK / STIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Applications for STIM1 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

5ug/ml

Western Blot

1ug/ml
Application Notes
This product is tested for ELISA and immunohistochemistry and suitable for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77kDa corresponding to the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (BSA)

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: STIM1

Stromal interaction molecule 1 plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. It acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, it translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit.

Long Name

Stromal Interaction Molecule 1

Alternate Names

GOK

Gene Symbol

STIM1

UniProt

Additional STIM1 Products

Product Documents for STIM1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for STIM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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