STING/TMEM173 Antibody (STING1/7431)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13941

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # STING1/7431

Concentration

0.2 mg/ml

View all STING/TMEM173 Antibodies »

Product Summary for STING/TMEM173 Antibody (STING1/7431)

Immunogen

Recombinant fragment (around aa190-290) of human STING/TMEM173 protein (exact sequence is proprietary) (Uniprot: Q86WV6 )

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14076)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.

Theoretical molecular weight: 37-50kDa

Scientific Data Images for STING/TMEM173 Antibody (STING1/7431)

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (STING1/7431) [NBP3-13941]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (STING1/7431) [NBP3-13941]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (STING1/7431) [NBP3-13941] - Formalin-fixed, paraffin-embedded human tonsil stained with STING/TMEM173 antibody (STING1/7431). Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (STING1/7431) [NBP3-13941]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (STING1/7431) [NBP3-13941]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (STING1/7431) [NBP3-13941] - Formalin-fixed, paraffin-embedded human tonsil stained with STING/TMEM173 antibody (STING1/7431). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
STING/TMEM173 Antibody (STING1/7431)

STING/TMEM173 Antibody (STING1/7431)

Western blot analysis of HDLM-2 cell lysate using STING/TMEM173 antibody (STING1/7431).

Applications for STING/TMEM173 Antibody (STING1/7431)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: STING/TMEM173

STING (stimulator of interferon genes) is encoded by the TMEM173 gene and is an adaptor molecule involved in the activation of innate immune responses to PAMPS (pathogen-associated molecular patterns) and DAMPS (damage-associated molecular patterns). STING specifically recognizes cytosolic DNA products derived from pathogens (e.g., cytomegalovirus, vaccinia virus, Listeria monocytogenes) or dead cells (1, 2). In the STING pathway, dsDNA derived from pathogens or damaged cells serves as a substrate for the enzyme cGAS (cyclic GMP-AMP synthase) which produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP (3, 4). Under steady-state conditions STING (theoretical molecular weight 42 kDa), a protein localizes to the ER membrane. Upon activation by dsDNA derived second messenger (cGAMP), STING translocates to the Golgi apparatus as a homodimer. Once STING has trafficked to the perinuclear region, it activates TANK binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3) and NF-kB leading to the production of cytokines (e.g., type I interferon) (2, 4). Mutations in the TMEM173 gene affecting STING expression are associated with the development of the auto-inflammatory disease SAVI (STING-associated vasculopathy with onset in infancy) (2). A novel SAVI dominant mutation in the TMEM173 human gene (V155M) leads to increased localization of STING to the Golgi and perinuclear region, indicative of an activated state (1). Hallmarks of SAVI, a rare inflammatory disease, include severe vasculitis in extremities and lung inflammation (7).

References

1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9

2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168

3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004

4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813

5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331

Long Name

Stimulator of Interferon Genes Protein/Transmembrane protein 173

Alternate Names

ERIS, MITA, MPYS, NET23, TMEM173

Gene Symbol

STING1

UniProt

Q86WV6 (Human)

Additional STING/TMEM173 Products

Product Documents for STING/TMEM173 Antibody (STING1/7431)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for STING/TMEM173 Antibody (STING1/7431)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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