STING/TMEM173 Antibody (STING1/7431) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14076
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # STING1/7431
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa190-290) of human STING/TMEM173 protein (exact sequence is proprietary) (Uniprot: Q86WV6 )
Localization
Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13941).
Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
Theoretical molecular weight: 37-50kDa
Antibody without azide - store at -20 to -80C. Antibody with azide - store at 2 to 8C. Non-hazardous.
Theoretical molecular weight: 37-50kDa
Scientific Data Images for STING/TMEM173 Antibody (STING1/7431) - Azide and BSA Free
STING/TMEM173 Antibody (STING1/7431)
Western blot analysis of HDLM-2 cell lysate using STING/TMEM173 antibody (STING1/7431).STING/TMEM173 Antibody (STING1/7431)
Formalin-fixed, paraffin-embedded human tonsil stained with STING/TMEM173 antibody (STING1/7431). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min .STING/TMEM173 Antibody (STING1/7431)
Formalin-fixed, paraffin-embedded human tonsil stained with STING/TMEM173 antibody (STING1/7431).Inset: PBS instead of primary antibody; secondary only negative controlApplications for STING/TMEM173 Antibody (STING1/7431) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: STING/TMEM173
References
1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9
2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168
3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004
4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813
5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331
Long Name
Stimulator of Interferon Genes Protein/Transmembrane protein 173
Alternate Names
ERIS, MITA, MPYS, NET23, TMEM173
Gene Symbol
STING1
Additional STING/TMEM173 Products
Product Documents for STING/TMEM173 Antibody (STING1/7431) - Azide and BSA Free
Product Specific Notices for STING/TMEM173 Antibody (STING1/7431) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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