SUB1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-59775
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Predicted:
Chicken (100%), Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 77 and 127 of human positive cofactor 4 using the numbering given in entry NP_006704.2 (GeneID 10923).
Reactivity Notes
Orangutan (100%). Rat reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SUB1 Antibody
![Western Blot: SUB1 Antibody [NB100-59775]](https://resources.bio-techne.com/images/products/SUB1-Antibody-Western-Blot-NB100-59775-img0010.jpg "Western Blot: SUB1 Antibody [NB100-59775]")
Western Blot: SUB1 Antibody [NB100-59775]
Western Blot: SUB1 Antibody [NB100-59775] - Analysis using rat cardiomyocyte lysate with anti-SUB1 antibody. Image from verified customer review.![Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775]](https://resources.bio-techne.com/images/products/SUB1-Antibody-Immunohistochemistry-Paraffin-NB100-59775-img0008.jpg "Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775]")
Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775]
Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PC4 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB![Immunoprecipitation: SUB1 Antibody [NB100-59775]](https://resources.bio-techne.com/images/products/SUB1-Antibody-Immunoprecipitation-NB100-59775-img0009.jpg "Immunoprecipitation: SUB1 Antibody [NB100-59775]")
Immunoprecipitation: SUB1 Antibody [NB100-59775]
Immunoprecipitation: SUB1 Antibody [NB100-59775] - Detection of human SUB1/PC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUB1 antibody NB100-59775 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B). SUB1 was immunoprecipitated by rabbit anti-SUB1 antibody NB100-59774, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).Applications for SUB1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Reviewed Applications
Read 1 review rated 5 using NB100-59775 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: SUB1
Alternate Names
activated RNA polymerase II transcription cofactor 4, activated RNA polymerase II transcriptional coactivator p15, MGC102747, p15, PC4p14P15, Positive cofactor 4, RPO2TC1, SUB1 homolog, SUB1 homolog (S. cerevisiae)
Entrez Gene IDs
10923 (Human)
Gene Symbol
SUB1
UniProt
Additional SUB1 Products
Product Documents for SUB1 Antibody
Product Specific Notices for SUB1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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