SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45683

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI6B11

Concentration

1 mg/ml

View all SUMO Activating Enzyme E1 (SAE1/UBA2) Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SAE1 (NP_005491) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) [NBP2-45683]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) [NBP2-45683]

Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUMO Activating Enzyme E1.
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) [NBP2-45683]

Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) [NBP2-45683]

Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) [NBP2-45683]

Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) [NBP2-45683]

Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (6B11) [NBP2-45683] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2

Long Name

SUMO1 Activating Enzyme E1

Alternate Names

AOS1, SAE1, SUA1, UBA2, UBLE1A

Gene Symbol

SAE1

Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products

Product Documents for SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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