SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74410
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0003.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Western-Blot-NBP2-74410-img0001.jpg "Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)Azide and BSA Free [NBP2-74410]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI6B11
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human SAE1 (NP_005491) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11)Azide and BSA Free [NBP2-74410]
Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUMO Activating Enzyme E1.![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0022.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0002.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]
Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0004.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0005.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0006.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0007.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0008.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0009.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0010.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0011.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0012.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0013.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0014.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0015.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0016.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0017.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0018.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0019.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0020.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0021.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
![Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]](https://resources.bio-techne.com/images/products/SUMO-Activating-Enzyme-E1-SAE1-Antibody-OTI6B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74410-img0023.jpg "Immunohistochemistry: SUMO Activating Enzyme E1 (SAE1) Antibody (OTI6B11) - Azide and BSA Free [NBP2-74410]")
Applications
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SUMO Activating Enzyme E1 (SAE1/UBA2)
Long Name
SUMO1 Activating Enzyme E1
Alternate Names
AOS1, SAE1, SUA1, UBA2, UBLE1A
Gene Symbol
SAE1
Additional SUMO Activating Enzyme E1 (SAE1/UBA2) Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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