Survivin Antibody (60.11) - Unpurified
Novus Biologicals, part of Bio-Techne | Catalog # NB500-205
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Proximity Ligation Assay, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 60.11
Format
Unpurified
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
This Survivin Antibody (60.11) was developed against full length recombinant human Survivin [UniProt# O15392].
Epitope
The epitope recognized is between amino acids 57-67.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 30572639)
Localization
Cytoplasmic
Specificity
Survivin Antibody (60.11) [NB500-205] is specific for the cytoplasmic form of survivin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Survivin Antibody (60.11) - Unpurified
Western Blot: Survivin Antibody (60.11)Unpurified [NB500-205]
Western Blot: Survivin Antibody (60.11) - Unpurified [NB500-205] - Survivin detected in rat aorta smooth muscle cell lysate in western blot analysis using [NB500-205]. Theoretical molecular weight: 16 kDa.Immunocytochemistry/ Immunofluorescence: Survivin Antibody (60.11) - Unpurified [NB500-205]
Immunocytochemistry/Immunofluorescence: Survivin Antibody (60.11) - Unpurified [NB500-205] - HeLa cells stained with Survivin Antibody (60.11) - Unpurified [NB500-205] (Green) and detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).Immunohistochemistry-Paraffin: Survivin Antibody (60.11) - Unpurified [NB500-205]
Immunohistochemistry-Paraffin: Survivin Antibody (60.11) - Unpurified [NB500-205] - Immunohistochemical staining of formalin-fixed paraffin-embedded ovarian cancer tissue using Survivin Antibody (60.11) - Unpurified [NB500-205].Applications for Survivin Antibody (60.11) - Unpurified
Application
Recommended Usage
Flow Cytometry
1:200
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry-Frozen
reported in scientific literature (PMID 22841823)
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 18332262)
Immunoprecipitation
1:10-1:500
Proximity Ligation Assay
reported in scientific literature (PMID 28077791)
Western Blot
reported in scientific literature (PMID 18332262)
Application Notes
This antibody weakly detects Survivin in Western blot; for this application we suggest using NB500-235 instead. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites with PBS
Format
Unpurified
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Survivin
Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).
Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).
/>References
/> 1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993
2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829
3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431
Alternate Names
API4, BIRC5
Gene Symbol
BIRC5
UniProt
Additional Survivin Products
Product Documents for Survivin Antibody (60.11) - Unpurified
Product Specific Notices for Survivin Antibody (60.11) - Unpurified
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...