Survivin Antibody (60.11) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-238
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Proximity Ligation Assay, Western Blot
Cited:
ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 60.11
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This Survivin Antibody (60.11) [Alexa Fluor 350] was developed against full length recombinant human Survivin [UniProt# O15392]
Epitope
The epitope recognized is between amino acids 27-67
Localization
Cytoplasmic
Specificity
Survivin Antibody (60.11) [NB500-238] is specific for the cytoplasmic form of Survivin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Survivin Antibody (60.11) - BSA Free
Immunohistochemistry: Survivin Antibody (60.11) - BSA Free [NB500-238]
Immunohistochemistry: Survivin Antibody (60.11) [NB500-238] - Low-power image of human high-grade chondrosarcoma displays strong cellular expression of survivin protein (A). High-power magnification reveals the predominantly cytoplasmic staining, although strong nuclear signals are detectable (B). Other specimen of a grade III chondrosarcoma stained with monoclonal antibody, shows a similar pattern of staining (C and D). For A and B the polyclonal rabbit anti-survivin antibody [AF886] was used. For C and D the monoclonal mouse Survivin Antibody (60.11) [NB500-238] was used. Image collected and cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license.Flow (Intracellular): Survivin Antibody (60.11) - BSA Free [NB500-238]
Flow (Intracellular): Survivin Antibody (60.11) [NB500-238] - An intracellular stain was performed on A549 cells with Survivin Antibody (60.11) [NB500-238] and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image using the Unpurified form of this antibody.Western Blot: Survivin Antibody (60.11)BSA Free [NB500-238]
Western Blot: Survivin Antibody (60.11) [NB500-238] - Survivin detection in 30ug of HeLa whole cell extract using [NB500-238] at 1ug/ml. Detected at predicted molecular weight of 16 kDa.Applications for Survivin Antibody (60.11) - BSA Free
Application
Recommended Usage
ELISA
reported in scientific literature (PMID 11861764)
Flow Cytometry
1 ug/mL. Use reported in scientific literature (PMID 25050620)
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Proximity Ligation Assay
reported in scientific literature (PMID 28077791)
Western Blot
1:1000
Application Notes
By WB, this antibody recognizes a band at ~16.5 kDa representing Survivin.
Reviewed Applications
Read 3 reviews rated 3.7 using NB500-238 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Survivin
Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).
Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).
/>References
/> 1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993
2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829
3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431
Alternate Names
API4, BIRC5
Gene Symbol
BIRC5
Additional Survivin Products
Product Documents for Survivin Antibody (60.11) - BSA Free
Product Specific Notices for Survivin Antibody (60.11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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