Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 197C193 (IM193)

Format

BSA Free

Concentration

1.0 mg/ml

View all TEM7/PLXDC1 Antibodies »

Product Specifications

Immunogen

Amino acids 409-425 (LQNNLSPKTKGTPVHLG) of human TEM7 were used to develop this monoclonal antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193))BSA Free [NB100-56557]

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193))BSA Free [NB100-56557]

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Analysis of human breast cancer lysate (35 ug per lane, RIPA buffer) at 0.03ug/ml. Band observed at ~60kDa. (Expected MW of 55.8kDa according to NP_065138.2).
Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free [NB100-56557]

Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free [NB100-56557]

Immunohistochemistry: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Staining of Tumor Endothelial Marker 7 in formalin-fixed, paraffin-embedded human Purkinje neurons at 2.5 ug/ml. Hematoxylin-eosin counterstain.
Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193))BSA Free [NB100-56557]

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193))BSA Free [NB100-56557]

Western Blot: TEM7/PLXDC1 Antibody (197C193 (IM193)) [NB100-56557] - Detection of TEM7 with TEM7 antibody. Human HCT-116 cell lysate probed with TEM7 antibody at 1 ug/ml.
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Applications for TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (Meng et al (2007))

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

reported in scientific literature (Lee et al (2006))

Immunohistochemistry-Paraffin

2-5 ug/ml

Immunoprecipitation

1:10-1:500. Use reported in scientific literature (Nanda et al (2004))
Application Notes
Immunohistochemistry-Paraffin reported in scientific literature (Nanda et al (2004); Lee et al (2005))
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TEM7/PLXDC1

Recently, using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al, have identified 46 genes, whose expression is specifically elevated in tumor-associated endothelium. Nine of these genes were prominently expressed only in tumor endothelial cells (EC), but were absent or barely detectable in normal ECs, and named as Tumor Endothelial Markers (TEMs, TEM 1-9). TEM7 (Tumor endothelial marker 7) transcripts are specifically expressed in the endothelium of colorectal cancer, primary cancers of lung, pancreas, breast, and brain. TEM7 is expressed specifically in endothelium of these cancers, whether primary or metastasis. The other six members of this family (TEM1, 3, 4, 5, 8, and 9) also show similar expression pattern in lung and brain tumors, and liver metastasis. Since most of the genes expressed differentially in tumor endothelium are also expressed during angiogenesis, these newly discovered genes might provide important resources for basic and clinical studies of human angiogenesis.

Long Name

Tumor Endothelial Marker 7/Plexin Domain Containing Protein 1

Alternate Names

PLXDC1, TEM3

Entrez Gene IDs

57125 (Human)

Gene Symbol

PLXDC1

UniProt

Q8IUK5 (Human)

Additional TEM7/PLXDC1 Products

Product Documents for TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TEM7/PLXDC1 Antibody (197C193 (IM193)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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