I would like to see image of WB to see the isoforms of the tenascin NB110-68136.

I am afraid we do not have notes or images on the westerns for this antibody. There are 2 publications that do have a double band in westerns for this antibody: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680762/. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1868127/. We have 2 Tenascin C antibodies that have been validated in mouse for western blot but none with images.

One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?

Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

Tenascin C Antibody (4C8MS) - BSA Free

Name: Tenascin C Antibody (4C8MS) - BSA Free
Brand: Novus Biologicals
SKU: NB110-68136
Rating: 4 (2 reviews)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-68136

(2)

Citations (26)

Product Datasheet / COA / SDS / Protocols & Manuals

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB110-68136AF350

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Alexa Fluor 405

NB110-68136AF405

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Alexa Fluor 488

NB110-68136AF488

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Alexa Fluor 532

NB110-68136AF532

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Alexa Fluor 594

NB110-68136AF594

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Alexa Fluor 647

NB110-68136AF647

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Alexa Fluor 700

NB110-68136AF700

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Alexa Fluor 750

NB110-68136AF750

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Allophycocyanin

NB110-68136APC

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Allophycocyanin/Cy7

NB110-68136APCCY7

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Biotin

NB110-68136B

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CoraFluor 1

NB110-68136CL1

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DyLight 350

NB110-68136UV

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DyLight 405

NB110-68136V

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DyLight 488

NB110-68136G

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DyLight 550

NB110-68136R

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DyLight 594

NB110-68136DL594

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DyLight 650

NB110-68136C

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DyLight 680

NB110-68136FR

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DyLight 755

NB110-68136IR

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FITC

NB110-68136F

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HRP

NB110-68136H

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Janelia Fluor 525

NB110-68136JF525

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Janelia Fluor 549

NB110-68136JF549

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Janelia Fluor 585

NB110-68136JF585

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Janelia Fluor 635

NB110-68136JF635

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Janelia Fluor 646

NB110-68136JF646

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Janelia Fluor 669

NB110-68136JF669

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mFluor Violet 450 SE

NB110-68136MFV450

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mFluor Violet 500 SE

NB110-68136MFV500

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mFluor Violet 610 SE

NB110-68136MFV610

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NB110-68136PE

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PE/Atto594

NB110-68136PEATT594

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PE/Cy5.5

NB110-68136PECY55

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PE/Cy7

NB110-68136PECY7

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Forumulation

Catalog #

Azide and BSA Free

NBP2-80973

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Feline

Cited:

Human, Mouse, Rat

Applications

Validated:

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA, Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4C8MS

Format

BSA Free

Concentration

1.0 mg/ml

View all Tenascin C Antibodies »

Product Specifications

Immunogen

Recombinant human Tenascin C [Swiss-Prot# P24821].

Reactivity Notes

Feline reactivity reported in customer review.

Localization

Secreted > extracellular space > extracellular matrix

Specificity

NB110-68136 specifically reacts with Domain B on FNIII repeats of Tenascin C.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Tenascin C Antibody (4C8MS) - BSA Free

Flow (Intracellular): Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].

Western Blot: Tenascin C Antibody (4C8MS)BSA Free [NB110-68136]

Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) [NB110-68136] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) [NB110-68136] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The antibody generated a very specific cytoplasmic, membrane and extra-cellular signal in tendon fibroblasts, osteoblasts, osteoclasts, and some bone marrow cells. The mineralized areas were largely negative for the staining.

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136] - An intracellular stain was performed on MCF7 cells with Tenascin C Antibody (4C8MS) NB110-68136 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) [NB110-68136] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

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Applications for Tenascin C Antibody (4C8MS) - BSA Free

Application

Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

5 ug/ml

Application Notes

For use in IHC-P, it is recommended to incubate primary antibody for at least 2 hours at room temperature followed by ON incubation at 4C. This antibody is CyTOF ready.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using NB110-68136 in the following applications:

Immunocytochemistry (1 Review)
Immunohistochemistry-Frozen (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tenascin C

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Alternate Names

Cytotactin, HXB, Tenascin J1, TNC

Entrez Gene IDs

3371 (Human); 116640 (Rat)

Gene Symbol

TNC

UniProt

P24821 (Human)

Additional Tenascin C Products

Product Documents for Tenascin C Antibody (4C8MS) - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download IHC-P Protocol

COA

SDS

Download SDS

Product Specific Notices for Tenascin C Antibody (4C8MS) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Protocols
FAQs

Tenascin C Antibody (4C8MS) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-68136

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Tenascin C Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Specificity

Clonality

Host

Isotype

Scientific Data Images for Tenascin C Antibody (4C8MS) - BSA Free

Flow (Intracellular): Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Western Blot: Tenascin C Antibody (4C8MS)BSA Free [NB110-68136]

Immunocytochemistry/ Immunofluorescence: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Flow Cytometry: Tenascin C Antibody (4C8MS) - BSA Free [NB110-68136]

Applications for Tenascin C Antibody (4C8MS) - BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Tenascin C

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Tenascin C Products

Product Documents for Tenascin C Antibody (4C8MS) - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for Tenascin C Antibody (4C8MS) - BSA Free