CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4C8MS

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Tenascin C Antibodies »

Product Summary for Tenascin C Antibody (4C8MS) - Azide and BSA Free

Immunogen

Recombinant human Tenascin C [Swiss-Prot# P24821].

Reactivity Notes

Feline reactivity reported in customer review.

Localization

Secreted > extracellular space > extracellular matrix

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Tenascin C Antibody (4C8MS) - Azide and BSA Free

Immunocytochemistry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Immunocytochemistry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The a

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse bone-tendon using Tenascin C antibody (clone 4C8MS) at 1:25 dilution. The signal was detected using HRP-DAB detection method which followed counterstaining using hematoxylin. The a

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Tenascin C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

Immunocytochemistry/ Immunofluorescence: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Immunocytochemistry/Immunofluorescence: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Tenascin C at 5 ug/ml overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody (4C8MS) NB110-68136AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973] - An intracellular stain was performed on SK-MEL-28 cells with Tenascin C Antibody [4C8MS] NB110-68136AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.

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Applications for Tenascin C Antibody (4C8MS) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (PMID 21871891)

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

reported in scientific literature (PMID 27256716)

Western Blot

5 ug/ml

Application Notes

For use in IHC-P, it is recommended to incubate primary antibody for at least 2 hours at room temperature followed by ON incubation at 4C. This antibody is CyTOF ready.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tenascin C

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Alternate Names

Cytotactin, HXB, Tenascin J1, TNC

Gene Symbol

TNC

Additional Tenascin C Products

Product Documents for Tenascin C Antibody (4C8MS) - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Tenascin C Antibody (4C8MS) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

Tenascin C Antibody (4C8MS) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80973

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Format

Concentration

View all Tenascin C Antibodies »

Product Summary for Tenascin C Antibody (4C8MS) - Azide and BSA Free

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Scientific Data Images for Tenascin C Antibody (4C8MS) - Azide and BSA Free

Immunocytochemistry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Immunohistochemistry-Paraffin: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Immunocytochemistry/ Immunofluorescence: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Flow Cytometry: Tenascin C Antibody (4C8MS) - Azide and BSA Free [NBP2-80973]

Applications for Tenascin C Antibody (4C8MS) - Azide and BSA Free

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Tenascin C

Alternate Names

Gene Symbol

Additional Tenascin C Products

Product Documents for Tenascin C Antibody (4C8MS) - Azide and BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Tenascin C Antibody (4C8MS) - Azide and BSA Free