TfR (Transferrin R) Antibody (DF1513)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32945

Clone DF1513 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-32945-0.02mg
NBP2-32945-0.1mg
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DF1513

Concentration

0.2 mg/ml

View all TfR (Transferrin R) Antibodies »

Product Specifications

Immunogen

KG1 acute myeloid leukemia cell line

Localization

Cell surface

Specificity

It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34602)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TfR (Transferrin R) Antibody (DF1513)

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Western Blot: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945]

Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) [NBP2-32945] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).
View 2 more images

Applications for TfR (Transferrin R) Antibody (DF1513)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Simple Western

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-32945 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TfR (Transferrin R)

Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity.

Long Name

Transferrin Receptor

Alternate Names

CD71, TfR (TransferrinR), TFR1, TFRC, TRFR

Gene Symbol

TFRC

UniProt

P02786 (Human)

Additional TfR (Transferrin R) Products

Product Documents for TfR (Transferrin R) Antibody (DF1513)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TfR (Transferrin R) Antibody (DF1513)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.