TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34602
Clone DF1513 was used by HLDA to establish CD designation.
![Simple Western: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Simple-Western-NBP2-34602-img0004.jpg "Simple Western: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]")
![Simple Western: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Simple-Western-NBP2-34602-img0005.jpg "Simple Western: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]")
![Western Blot: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Western-Blot-NBP2-34602-img0007.jpg "Western Blot: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # DF1513
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free
Immunogen
KG1 acute myeloid leukemia cell line
Localization
Cell surface
Specificity
It recognizes a ~90-95kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190kDa (Workshop IV). This monoclonal antibody is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32945).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free
Western Blot: TfR (Transferrin R) Antibody (DF1513)Azide and BSA Free [NBP2-34602]
Western Blot: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Western Blot Analysis of Jurkat, Raji, and THP-1 cell lysate using TfR (Transferrin R) Antibody (DF1513).![Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34602-img0006.jpg "Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]")
Immunocytochemistry/ Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]
Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Immunofluorescence Analysis of Human Jurkat cells labeling CD71 with TfR (Transferrin R) Antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)![Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]](https://resources.bio-techne.com/images/products/TfR-Transferrin-R-Antibody-DF1513-Azide-and-BSA-Free-Flow-Cytometry-NBP2-34602-img0008.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]")
Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602]
Flow Cytometry: TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free [NBP2-34602] - Flow Cytometric Analysis of human Jurkat cells using TfR (Transferrin R) antibody (DF1513) followed by Goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).Applications for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Western Blot
0.25-0.5ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: TfR (Transferrin R)
Long Name
Transferrin Receptor
Alternate Names
CD71, TfR (TransferrinR), TFR1, TFRC, TRFR
Gene Symbol
TFRC
Additional TfR (Transferrin R) Products
Product Documents for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free
Product Specific Notices for TfR (Transferrin R) Antibody (DF1513) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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