TGF-beta 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AB-112-NA
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Multi-Species
Cited:
Human, Mouse
Applications
Validated:
Neutralization, Simple Western, Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Porcine platelet-derived TGF-beta 2
Specificity
Detects TGF-beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody is highly specific for TGF-beta 2 andTGF‑ beta1.2 but will cross-react with other TGF-beta variants with at least 100-fold lower sensitivity. It will also neutralize the biological activity of TGF-beta 1.2, at a 50 fold higher IgG concentration.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for TGF-beta 2 Antibody
Detection of Human TGF‑ beta2 by Western Blot.
Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-112-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF-beta 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Detection of Human TGF‑ beta2 by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF-beta 2 at approximately 63 kDa (as indicated) using 2.5 µg/mL of Goat Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-112-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.TGF‑ beta2 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta2 Antibody.
Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-112-NA). The ND50 is typically 0.3-1.5 µg/mL.Applications for TGF-beta 2 Antibody
Application
Recommended Usage
Simple Western
2.5 µg/mL
Sample: Human heart tissue and human breast cancer tissue
Sample: Human heart tissue and human breast cancer tissue
Western Blot
0.25 µg/mL
Sample: Human heart tissue and human breast cancer tissue
Sample: Human heart tissue and human breast cancer tissue
Neutralization
Measured by its ability to neutralize TGF‑ beta2 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.3-1.5 µg/mL in the presence of 1 ng/mL Porcine TGF‑ beta2 and 7.5 ng/mL Recombinant Mouse IL‑4.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta 2
References
- Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
- Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
- Wahl, S.M. (2006) Immunol. Rev. 213:213.
- Chang, H. et al. (2002) Endocr. Rev. 23:787.
- Lin, J.S. et al. (2006) Reproduction 132:179.
- Hinck, A.P. et al. (1996) Biochemistry 35:8517.
- Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
- deMartin, R. et al. (1987) EMBO J. 6:3673.1.
- Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
- Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
- de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
- Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name
Transforming Growth Factor beta 2
Alternate Names
TGFB2, TGFbeta 2
Gene Symbol
TGFB2
Additional TGF-beta 2 Products
Product Documents for TGF-beta 2 Antibody
Product Specific Notices for TGF-beta 2 Antibody
For research use only
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