Thimet Oligopeptidase/THOP1 Antibody (4D6)
Novus Biologicals, part of Bio-Techne | Catalog # NB400-146
![Western Blot: Thimet Oligopeptidase/THOP1 Antibody (4D6) [NB400-146]](https://resources.bio-techne.com/images/products/Thimet-Oligopeptidase-Antibody-4D6-Western-Blot-NB400-146-img0003.jpg "Western Blot: Thimet Oligopeptidase/THOP1 Antibody (4D6) [NB400-146]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate, Rabbit
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 4D6
Concentration
1.54 mg/ml
Product Specifications
Immunogen
A synthetic peptide corresponding to residues 56-72 of human Thimet Oligopeptidase. [UniProt# P52888]
Localization
Cytoplasmic
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Thimet Oligopeptidase/THOP1 Antibody (4D6)
Western Blot: Thimet Oligopeptidase/THOP1 Antibody (4D6) [NB400-146]
Western Blot: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Detection of thimet oligopeptidase antibody in HeLa whole cell lysate (30 ug) using NB400-146. Lane 1: 0.5 ug/ml; lane 2: 2 ug/ml. 1 minute ECL exposure.![Immunohistochemistry: Thimet Oligopeptidase/THOP1 Antibody (4D6) [NB400-146]](https://resources.bio-techne.com/images/products/Thimet-Oligopeptidase-Antibody-4D6-Immunohistochemistry-NB400-146-img0004.jpg "Immunohistochemistry: Thimet Oligopeptidase/THOP1 Antibody (4D6) [NB400-146]")
Immunohistochemistry: Thimet Oligopeptidase/THOP1 Antibody (4D6) [NB400-146]
Immunohistochemistry: Thimet Oligopeptidase Antibody (4D6) [NB400-146] - Analysis of Thimet Oligopeptidase in mouse brain using DAB with hematoxylin counterstain.Applications for Thimet Oligopeptidase/THOP1 Antibody (4D6)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:400
Immunohistochemistry-Paraffin
1:400
Western Blot
1:750-1:3000
Application Notes
This Thimet Oligopeptidase (4D6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~80 kDa. For IHC-P, this antibody has been tested in human, monkey, and rat brain and testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.54 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Thimet Oligopeptidase/THOP1
Additional Thimet Oligopeptidase/THOP1 Products
Product Documents for Thimet Oligopeptidase/THOP1 Antibody (4D6)
Product Specific Notices for Thimet Oligopeptidase/THOP1 Antibody (4D6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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