Thimet Oligopeptidase/THOP1 Antibody (4D6) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB400-146F

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate, Rabbit

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 4D6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Thimet Oligopeptidase/THOP1 Antibodies »

Product Summary for Thimet Oligopeptidase/THOP1 Antibody (4D6) [FITC]

Immunogen

A synthetic peptide corresponding to residues 56-72 of human Thimet Oligopeptidase. [UniProt# P52888]

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Thimet Oligopeptidase/THOP1 Antibody (4D6) [FITC]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Thimet Oligopeptidase/THOP1

Thimet oligopeptidase (THOP1) is a thiol-rich enzyme with a HEXXH catalytic motif containing zinc distributed ubiquitously in mammalian cells and it belongs to family of M3 metallopeptidases. THOP1 is an oligopeptidase, as it only degrades short peptides (5-17 amino acids) and because of thiol compounds mediated activation, it is referred to as thimet. THOP1 implicates in degradation of peptides released by the 26 S proteasome and is prone to oxidative oligomerization through formation of interprotein disulfides involving specific Cys residues. Maximal THOP1 activity is maintained by partial S-glutathionylation, which is a mechanism that regulates protein oligomerization and enzymatic activity. Besides exising as a secreted neuropeptide-metabolizing enzyme, it is predominately expressed in cytoplasm and nucleus of neuronal cells. THOP1 is involved in cytoplasmic peptide degradation and cleaves bioactive peptides inside and outside CNS (including degradation of beta-amyloid precursor protein and generation of amyloidogenic fragments). It has also been shown to hydrolyses bradykinin (BK), neurotensin (NT), GnRH (gonadotrophin-release hormone), beta-neoendorphin, alpha-neoendorphin, dynorphin 1-8 and nociceptin/orphanin in vitro. THOP1 activity may be controlled by phosphorylation and/or by intermolecular disulfide bonds leading to inactive oligomers, and it expression is differentially regulated in neuroendocrine and spermatid cell lineages by a transcription factor binding to SRY (sex-determining region Y), CAAT box and CREB (cAMP-response element-binding protein) promoter consensus sequences.

Alternate Names

EP24.15

Gene Symbol

THOP1

Additional Thimet Oligopeptidase/THOP1 Products

Product Documents for Thimet Oligopeptidase/THOP1 Antibody (4D6) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Thimet Oligopeptidase/THOP1 Antibody (4D6) [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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