Tie-2 Antibody (tek9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18614

Novus Biologicals, part of Bio-Techne
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NBP1-18614
NBP1-18614-0.02mg
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Sandwich ELISA, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # tek9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Tie-2 Antibodies »

Product Specifications

Immunogen

Recombinant human soluble extracellular TIE-2

Specificity

The monoclonal antibody will detect native human TIE-2/tek in ELISA experiments and on the surface of different human cell types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tie-2 Antibody (tek9) - Azide and BSA Free

Flow Cytometry: Tie-2 Antibody (tek9) - Azide and BSA Free [NBP1-18614]

Flow Cytometry: Tie-2 Antibody (tek9) - Azide and BSA Free [NBP1-18614]

Flow Cytometry: Tie-2 Antibody (tek9) [NBP1-18614] - FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).
Sandwich ELISA: Tie-2 Antibody (tek9) [NBP1-18614] - Quantification of soluble and cellular Tie-2 by sandwich ELISA. CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTie-2 or Tie-2. For capturing anti-human Tie-2 Cl.16 NBP1-18616 was used, for the detection of a mixture of biotinylated anti-human Tie-2 Cl.2 NBP1-18612 and Cl.9 NBP1-18614.

Applications for Tie-2 Antibody (tek9) - Azide and BSA Free

Application
Recommended Usage

ELISA

1 - 2 ug/ml

Flow Cytometry

2 - 5 ug/ml

Western Blot

2 - 5 ug/ml
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tie-2

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Alternate Names

CD202b, TEK, Tie2

Gene Symbol

TEK

Additional Tie-2 Products

Product Documents for Tie-2 Antibody (tek9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tie-2 Antibody (tek9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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