Tie-2 Antibody (tek9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18614
![Flow Cytometry: Tie-2 Antibody (tek9) - Azide and BSA Free [NBP1-18614]](https://resources.bio-techne.com/images/products/Tie-2-Antibody-tek9-Flow-Cytometry-NBP1-18614-img0014.jpg "Flow Cytometry: Tie-2 Antibody (tek9) - Azide and BSA Free [NBP1-18614]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, ELISA, Flow Cytometry, Sandwich ELISA, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # tek9
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Recombinant human soluble extracellular TIE-2
Specificity
The monoclonal antibody will detect native human TIE-2/tek in ELISA experiments and on the surface of different human cell types.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Tie-2 Antibody (tek9) - Azide and BSA Free
Flow Cytometry: Tie-2 Antibody (tek9) - Azide and BSA Free [NBP1-18614]
Flow Cytometry: Tie-2 Antibody (tek9) [NBP1-18614] - FACS analysis with primary human dermal microvascular endothelial cells (HDMVEC).
Sandwich ELISA: Tie-2 Antibody (tek9) [NBP1-18614] - Quantification of soluble and cellular Tie-2 by sandwich ELISA. CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTie-2 or Tie-2. For capturing anti-human Tie-2 Cl.16 NBP1-18616 was used, for the detection of a mixture of biotinylated anti-human Tie-2 Cl.2 NBP1-18612 and Cl.9 NBP1-18614.
Applications for Tie-2 Antibody (tek9) - Azide and BSA Free
Application
Recommended Usage
ELISA
1 - 2 ug/ml
Flow Cytometry
2 - 5 ug/ml
Western Blot
2 - 5 ug/ml
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Tie-2
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Alternate Names
CD202b, TEK, Tie2
Gene Symbol
TEK
Additional Tie-2 Products
Product Documents for Tie-2 Antibody (tek9) - Azide and BSA Free
Product Specific Notices for Tie-2 Antibody (tek9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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