TIMP-2 Antibody (TIMP2/2044)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07720

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # TIMP2/2044

Concentration

0.2 mg/ml

View all TIMP-2 Antibodies »

Product Specifications

Immunogen

Recombinant human TIMP-2 protein fragment (aa 48-220) (exact sequence is proprietary) (Uniprot: P16035)

Localization

Cytoplasm and Cell Surface

Specificity

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08714)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TIMP-2 Antibody (TIMP2/2044)

Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]

Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]

Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720] - Formalin-fixed, paraffin-embedded human Placenta stained with TIMP-2 Mouse Monoclonal Antibody (TIMP-2/2044).
Protein Array: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]

Protein Array: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720]

Protein Array: TIMP-2 Antibody (TIMP2/2044) [NBP3-07720] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIMP-2 Mouse Monoclonal Antibody (TIMP-2/2044).

Applications for TIMP-2 Antibody (TIMP2/2044)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TIMP-2

The TIMP2 gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role a

Long Name

Tissue Inhibitors of Metalloproteinases 2

Alternate Names

TIMP2

Gene Symbol

TIMP2

UniProt

P16035

Additional TIMP-2 Products

Product Documents for TIMP-2 Antibody (TIMP2/2044)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIMP-2 Antibody (TIMP2/2044)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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