Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Guinea Pig, Rabbit, Zebrafish

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # TIMP2/2488R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all TIMP-2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide from the N-terminus of human TIMP-2 protein (aa56-66) (DSGNDIYGNPI) (Uniprot: P16035)

Localization

Cytoplasm and Cell Surface

Specificity

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07724).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TIMP-2 Antibody (TIMP2/2488R) - Azide and BSA Free

Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2488R) - Azide and BSA Free [NBP3-08721]

Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2488R) - Azide and BSA Free [NBP3-08721]

Immunohistochemistry-Paraffin: TIMP-2 Antibody (TIMP2/2488R) - Azide and BSA Free [NBP3-08721] - Formalin-fixed, paraffin-embedded human Placenta stained with TIMP-2 Rabbit Recombinant Monoclonal Antibody (TIMP-2/2488R).

Applications for TIMP-2 Antibody (TIMP2/2488R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TIMP-2

The TIMP2 gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role a

Long Name

Tissue Inhibitors of Metalloproteinases 2

Alternate Names

TIMP2

Gene Symbol

TIMP2

Additional TIMP-2 Products

Product Documents for TIMP-2 Antibody (TIMP2/2488R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIMP-2 Antibody (TIMP2/2488R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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