Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Rabbit

Cited:

Human, Canine

Applications

Validated:

Block/Neutralize, CyTOF-ready, ELISA, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Western Blot

Cited:

Block/Neutralize, Flow (Cell Surface), Flow Cytometry, Flow Cytometry Control, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, In vitro assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # TL2.1

Format

Azide Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLR2 Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TLR2 Antibody (TL2.1) - Azide Free

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using Azide/BSA FREE version of NB100-56726. Intracellular flow analysis of TLR2 in 1x10^6 PBMCs using 2 ug of NB100-56722. Shaded histogram represents cells without antibody; green represents isotype control (Sigma, Cat. No. M5409); purple represents anti-TLR2 antibody.
Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Intracellular analysis of TLR2 in 10^6 PBMs using 2 ug of TLR2 antibody. Shaded histogram represents cells without antibody; green represents isotype control ; red represents TLR2 antibody. goat anti-mouse IgG FITC secondary antibody was used in this test.
Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726]

Flow Cytometry: TLR2 Antibody (TL2.1) - Azide Free [NB100-56726] - Analysis using the FITC conjugate of NB100-56726. Staining of TLR2 in 10^6 PBMCs (lymphocyte-gated) using 2 ugs ofMouse IgG2a Kappa , and red represents TLR2 antibody.
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Applications for TLR2 Antibody (TL2.1) - Azide Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (Flo et al (2000); PMID 24512642)

ELISA

reported in scientific literature (PMID 23629653)

Flow (Cell Surface)

1-2 ug/1 million cells

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:2000. Use reported in scientific literature (Mempel et al (2003))

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 11160251)

Immunoprecipitation

1:10 - 1:500. Use reported in scientific literature (Flo et al (2000))

In vitro assay

reported in scientific literature (PMID 24512642)

Western Blot

reported in scientific literature (PMID 28546218)
Application Notes
Flow (Cell Surface) reported in scientific literature (PMID 24836676)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using NB100-56726 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Filter (0.2 micron) sterilized PBS

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR2

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody (TL2.1) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLR2 Antibody (TL2.1) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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