Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Rabbit

Cited:

Human, Canine, Rabbit

Applications

Validated:

ELISA, Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry, Flow Cytometry Control, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # TL2.1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLR2 Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry.

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 21273544).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TLR2 Antibody (TL2.1) [PE]

Flow Cytometry: TLR2 Antibody (TL2.1) [PE] [NBP2-24909] - Cell surface analysis of TLR2 on stable transfected cell line using this antibody at 1 ug/10^6 cells. Green represents isotype control ; red represents anti-TLR2 antibody.
Flow Cytometry: TLR2 Antibody (TL2.1) [PE] [NBP2-24909] - Cell surface analysis of TLR2 on PBMC using this antibody at 1 ug/10^6 cells. Green represents isotype control this antibody; red represents anti-TLR2 antibody.

Applications for TLR2 Antibody (TL2.1) [PE]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

2-5 ug/ml
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-24909 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TLR2

Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation.

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody (TL2.1) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLR2 Antibody (TL2.1) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.