Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse, Canine

Applications

Validated:

Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry, Flow Cytometry Control, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 85B152.5

Format

BSA Free

Concentration

1.0 mg/ml

View all TLR5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TLR5 Antibody (85B152.5) - BSA Free

Western Blot: TLR5 Antibody (85B152.5)BSA Free [NBP1-97728]

Western Blot: TLR5 Antibody (85B152.5)BSA Free [NBP1-97728]

Western Blot: TLR5 Antibody (85B152.5) [NBP1-97728] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.
Flow Cytometry: TLR5 Antibody (85B152.5) - BSA Free [NBP1-97728]

Flow Cytometry: TLR5 Antibody (85B152.5) - BSA Free [NBP1-97728]

Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the FITC conjugate of NBP1-97728. Staining of TLR5 in 10^6 cells using 0.5 ugs of FITC-conjugated antibody: A) Intracellular flow testing of human Ramos cells and B) cell surface flow testing of mouse RAW cells. The shaded (or black) histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.
Flow Cytometry: TLR5 Antibody (85B152.5) - BSA Free [NBP1-97728]

Flow Cytometry: TLR5 Antibody (85B152.5) - BSA Free [NBP1-97728]

Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in Ramos cells using 1 ug of TLR5 antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 antibody.
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Applications for TLR5 Antibody (85B152.5) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (Wong et al (2007); PMID 24779433)

Flow (Intracellular)

reported in scientific literature (PMID 24779433)

Flow Cytometry

1-3 ug/10^6 cells in 100 ul

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 17570691)

Western Blot

1-3 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR5

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Long Name

Toll-like Receptor 5

Alternate Names

TIL-3

Gene Symbol

TLR5

Additional TLR5 Products

Product Documents for TLR5 Antibody (85B152.5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLR5 Antibody (85B152.5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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