Key Product Details

Species Reactivity

Validated:

Human, Mouse, Canine

Cited:

Human, Mouse, Canine

Applications

Validated:

Flow (Cell Surface), Flow (Intracellular), Flow Cytometry

Cited:

Flow (Cell Surface), Flow Cytometry, Flow Cytometry Control

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 85B152.5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLR5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TLR5 Antibody (85B152.5) [PE]

Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - A surface stain was performed on A431 cells with TLR5 [85B152.5] Antibody NBP2-24959 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - Cell surface analysis of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.
Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

Applications for TLR5 Antibody (85B152.5) [PE]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TLR5

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Long Name

Toll-like Receptor 5

Alternate Names

TIL-3

Gene Symbol

TLR5

Additional TLR5 Products

Product Documents for TLR5 Antibody (85B152.5) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLR5 Antibody (85B152.5) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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