TMEM97 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30436

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TMEM97 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the human TMEM97 protein (within residues 105-155.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: crab-eating macaque (98%). Mouse reactivity reported in scientific literature (PMID: 30594810).

Localization

Membrane; Multi-pass membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TMEM97 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TMEM97 Antibody - BSA Free [NBP1-30436]

Immunocytochemistry/ Immunofluorescence: TMEM97 Antibody - BSA Free [NBP1-30436]

Immunocytochemistry/Immunofluorescence: TMEM97 Antibody [NBP1-30436] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-TMEM97 Antibody NBP1-30436 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Western Blot: TMEM97 AntibodyBSA Free [NBP1-30436]

Western Blot: TMEM97 AntibodyBSA Free [NBP1-30436]

Western Blot: TMEM97 Antibody [NBP1-30436] - Expression of proteins involved in extracellular lipid uptake and intracellular cholesterol trafficking following JQ1 (0.4 uM) administration to HepG2 cells for 48 hours. Representative Western blots and densitometric analysis of TMEM97. Tubulin was chosen as loading control. n = 6 independent experiments. Data represent means +/- SD. Statistical analysis was performed by using unpaired Student's t test. ** p < 0.01; *** p < 0.001. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/21/4/1297), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: TMEM97 Antibody - BSA Free [NBP1-30436]

Immunohistochemistry-Paraffin: TMEM97 Antibody - BSA Free [NBP1-30436]

Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP1-30436] - Analysis of a FFPE tissue section of human pancreas using 1:200 dilution of TMEM97 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.
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Applications for TMEM97 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-5 ug/ml

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

2 ug/ml
Application Notes
In Western blot, a band is seen at ~24 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TMEM97

TMEM97 (transmembrane protein 97 or protein MAC30) is a member of IGFBP/insulin-like growth factor-binding protein family and is implicated in several biological processes wherein it acts through its regulatory effects on IGF activity. It is a multi-pass membrane protein which is localized in nuclear and cell membranes, RER and lysosomes, and is expressed in several normal tissues including brain, lung, heart, skeletal muscles, testis, ovary, and pregnant uterus. TMEM97 was originally identified as overexpressed in meningiomas, however, later it was found to exhibit altered expression in different types of cancers. The absence or downregulation of TMEM97 expression in pancreatic cancer suggests that it may acts as a tumor suppressor in pancreatic cancer. Contrastingly, its expression is stronger in breast, esophageal, stomach, colon, and oral squamous cell cancers than the corresponding normal tissues. Its physiological function is largely unknown, however, it has been suggested to implicate in cellular proliferation/differentiation and regulation of cholesterol homeostasis.

Long Name

Sigma intracellular receptor 2

Alternate Names

MAC30, S2R, Sigma-2 receptor, Sigma2 receptor, anti

Entrez Gene IDs

27346 (Human); 69071 (Mouse)

Gene Symbol

TMEM97

UniProt

Q5BJF2 (Human), Q8VD00 (Mouse)

Additional TMEM97 Products

Product Documents for TMEM97 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TMEM97 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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