TRA-1-81 Antibody (TRA-1-81)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1833

Novus Biologicals, part of Bio-Techne
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NB100-1833
NB100-1833SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative)

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # TRA-1-81

Concentration

1 mg/ml

View all TRA-1-81 Antibodies »

Product Specifications

Immunogen

Human embryonal carcinoma cell line 2102Ep.

Reactivity Notes

This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.

Localization

Cell Membrane

Marker

Embryonic Stem Cell Marker, Tumor Vessel Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for TRA-1-81 Antibody (TRA-1-81)

Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]

Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]

Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate.
Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]

Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]

Immunocytochemistry/Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane.
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]

Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]

Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.

Applications for TRA-1-81 Antibody (TRA-1-81)

Application
Recommended Usage

Flow Cytometry

1:10-1:1000. Use reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Frozen

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRA-1-81

TRA-1-81 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Long Name

Tumor-related Antigen-1-81

Alternate Names

TRA181

Additional TRA-1-81 Products

Product Documents for TRA-1-81 Antibody (TRA-1-81)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRA-1-81 Antibody (TRA-1-81)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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