TRA-1-81 Antibody (TRA-1-81)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1833
![Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]](https://resources.bio-techne.com/images/products/TRA-1-81-Antibody-TRA-1-81-Western-Blot-NB100-1833-img0006.jpg "Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative)
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # TRA-1-81
Concentration
1 mg/ml
Product Specifications
Immunogen
Human embryonal carcinoma cell line 2102Ep.
Reactivity Notes
This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.
Localization
Cell Membrane
Marker
Embryonic Stem Cell Marker, Tumor Vessel Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for TRA-1-81 Antibody (TRA-1-81)
Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]
Western Blot: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate.![Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]](https://resources.bio-techne.com/images/products/TRA-1-81-Antibody-TRA-1-81-Immunocytochemistry-Immunofluorescence-NB100-1833-img0012.jpg "Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]")
Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]
Immunocytochemistry/Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane.![Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]](https://resources.bio-techne.com/images/products/TRA-1-81-Antibody-TRA-1-81-Flow-Cytometry-NB100-1833-img0010.jpg "Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]")
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833]
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [NB100-1833] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.Applications for TRA-1-81 Antibody (TRA-1-81)
Application
Recommended Usage
Flow Cytometry
1:10-1:1000. Use reported in scientific literature
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Frozen
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
IgM purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRA-1-81
Long Name
Tumor-related Antigen-1-81
Alternate Names
TRA181
Additional TRA-1-81 Products
Product Documents for TRA-1-81 Antibody (TRA-1-81)
Product Specific Notices for TRA-1-81 Antibody (TRA-1-81)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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