TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80986

Novus Biologicals, part of Bio-Techne
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NBP2-80986
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Key Product Details

Species Reactivity

Human, Mouse (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # TRA-1-81

Format

Azide and BSA Free

Concentration

1 mg/ml

View all TRA-1-81 Antibodies »

Product Specifications

Immunogen

Human embryonal carcinoma cell line 2102Ep.

Reactivity Notes

This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.

Localization

Cell Membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free

Western Blot: TRA-1-81 Antibody (TRA-1-81)Azide and BSA Free [NBP2-80986]

Western Blot: TRA-1-81 Antibody (TRA-1-81)Azide and BSA Free [NBP2-80986]

Western Blot: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]

Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]

Immunocytochemistry/Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Image from the standard format of this antibody.
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]

Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]

Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.

Applications for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000. Use reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Frozen

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

IgM purified

Formulation

Tris-Glycine, 0.15 M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRA-1-81

TRA-1-81 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Long Name

Tumor-related Antigen-1-81

Alternate Names

TRA181

Additional TRA-1-81 Products

Product Documents for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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