Tryptophan hydroxylase 2 Antibody (CL2990)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46646

Novus Biologicals, part of Bio-Techne
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NBP2-46646
NBP2-46646-25ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2990

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Tryptophan Hydroxylase 2 Antibodies »

Product Specifications

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence SITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDLNTVCDALNKMNQYLG

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tryptophan hydroxylase 2 Antibody (CL2990)

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of rat midbrain shows strong positivity in serotonin neurons in the dorsal raphe nucleus.
Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the basal forebrain.
Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646]

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the cerebral cortex.
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Applications for Tryptophan hydroxylase 2 Antibody (CL2990)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 2

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Alternate Names

NTPH, TPH2

Entrez Gene IDs

121278 (Human)

Gene Symbol

TPH2

UniProt

Q8IWU9 (Human)

Additional Tryptophan Hydroxylase 2 Products

Product Documents for Tryptophan hydroxylase 2 Antibody (CL2990)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Tryptophan hydroxylase 2 Antibody (CL2990)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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