Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43817

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-43817
Print Quote
Bulk Order
Forumulation
Catalog #
BSA Free
NBP2-46646
View Product
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2990

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Tryptophan Hydroxylase 2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q8IWU9, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free

Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NBP3-43817]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NBP3-43817]

Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.
Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NBP3-43817]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NBP3-43817]

Staining of human dorsal raphe nucleus shows strong cytoplasmic positivity in serotonin neurons.
Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NBP3-43817]

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NBP3-43817]

Staining of human cerebral cortex shows moderate positivity in serotonergic neural fibers.
View 3 more images

Applications for Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptophan Hydroxylase 2

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Alternate Names

NTPH, TPH2

Gene Symbol

TPH2

Additional Tryptophan Hydroxylase 2 Products

Product Documents for Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.