TSC2 Antibody (SC05-59)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67552
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SC05-59
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human TSC2 aa 1760-1807 / 1807. (SwissProt: P49815 Human; SwissProt: Q61037 Mouse; SwissProt: P49816 Rat)
Localization
Cytoplasm, Membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TSC2 Antibody (SC05-59)
Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552]
Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-RabImmunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552]
Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of paraffin-embedded mouse placenta tissue using anti-Tuberin antibody. Counter stained with hematoxylin.Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552]
Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Flow cytometric analysis of TSC2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).Applications for TSC2 Antibody (SC05-59)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TSC2
Long Name
Tuberous Sclerosis 2
Alternate Names
LAM, TSC4, Tuberin
Gene Symbol
TSC2
Additional TSC2 Products
Product Documents for TSC2 Antibody (SC05-59)
Product Specific Notices for TSC2 Antibody (SC05-59)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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