TSC2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76430

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all TSC2 Antibodies »

Product Summary for TSC2 Antibody - BSA Free

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human TSC2. The immunogen is located within amino acids 1450 - 1500 of TSC2. Amino Acid Squence: RRGKRVERDALKSR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TSC2 Antibody - BSA Free

Western Blot: TSC2 AntibodyBSA Free [NBP1-76430]

Western Blot: TSC2 AntibodyBSA Free [NBP1-76430]

Western Blot: TSC2 Antibody [NBP1-76430] - Western blot analysis of TSC2 in L1210 cell lysate with anti-TSC2 (CT) using IMG-5540 at 1 ug/ml in the (A) absence and (B) presence of blocking peptide.

Applications for TSC2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1-2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSC2

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 complex decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing that overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. At least three isoforms of TSC2 exist.

Long Name

Tuberous Sclerosis 2

Alternate Names

LAM, TSC4, Tuberin

Gene Symbol

TSC2

UniProt

NP_000539 (Human)

Additional TSC2 Products

Product Documents for TSC2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TSC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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