Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S15/4

Concentration

1 mg/ml

View all Vanilloid R-like 3/TRPV3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide amino acids 774-791 (C-terminus) of rat TrpV3

Localization

Membrane

Specificity

Detects approx 70 kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Vanilloid R-like 3/TRPV3 Antibody (S15/4)

Western Blot: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909]

Western Blot: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909]

Western Blot: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Western Blot analysis of Human Cell lysates showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909]

Immunocytochemistry/ Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909]

Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.
Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909]

Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909]

Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (S15/4) [NBP2-12909] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone N15/4 (NBP2-12909). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12909) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
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Applications for Vanilloid R-like 3/TRPV3 Antibody (S15/4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000
Application Notes
1 ug/ml of TrpV3 Antibody was sufficient for detection of TrpV3 in 10 ug of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vanilloid R-like 3/TRPV3

1 ug/mL was sufficient for detection of TrpV3 in 10ug of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

Vanilloid Receptor Like 3/Transient Receptor Potential Cation Channel 3

Alternate Names

TRPV3, VanilloidR like 3, VanilloidR-like3, VRL3

Gene Symbol

TRPV3

Additional Vanilloid R-like 3/TRPV3 Products

Product Documents for Vanilloid R-like 3/TRPV3 Antibody (S15/4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Vanilloid R-like 3/TRPV3 Antibody (S15/4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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