Vav-1 [p Tyr174] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13139

Novus Biologicals, part of Bio-Techne
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NBP3-13139
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Vav-1 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr174 of human Vav-1. The exact sequence is proprietary.

Modification

p Tyr 174

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Vav-1 [p Tyr174] Antibody

Western Blot: Vav-1 [p Tyr174] Antibody [NBP3-13139]

Western Blot: Vav-1 [p Tyr174] Antibody [NBP3-13139]

Western Blot: Vav-1 [p Tyr174] Antibody [NBP3-13139] - Untreated (-) and treated (+) Jurkat whole cell extracts (60 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Vav-1 Tyr 174 antibody (NBP3-13139) diluted at 1:1000.

Applications for Vav-1 [p Tyr174] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vav-1

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels (2). As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein (3). Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response (2).

Alternate Names

vav-T, Vav1

Gene Symbol

VAV1

Additional Vav-1 Products

Product Documents for Vav-1 [p Tyr174] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Vav-1 [p Tyr174] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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